One of the largest wildlife refuge areas in Lancaster County, PA is Millport Conservancy near Lititz borough, between Lititz and Rothsville, PA. Homes for sale in the area are few simply because the refuge takes up much of the valley that Lititz Run (another word for creek) flows through. 
The preserve is 100 acres of land along Lititz Run, beginning at Rothsville Road on the north and ending at Millport Road on the southern side. The area is a valley, with a picturesque winding road that parallels the Conservancy.
A mill sits up on the hill off the main road, and large pond lies across the center of the valley about midway down the run. Dense brush greets the traveller if you're not on an approved trail, so don't even try it!
The creek itself is also picturesque (see more photos here) with shaded, peaceful sections where all you can hear is the babbling water around you.
There are birds, frogs and fish everywhere - not to metion the wide variety of wildflowers. Being in this place no doubt gives us an idea of what Lancaster County was like before development pressures came to bear. Very serene.
The Millport Conservancy is part of the Growing Greener initiative of the PA Dept. of Environmental Protection (DEP) and receives funds to aid in preservation efforts. The Conservancy is a key component of the Lititz Watershed Alliance, a non-profit corporation of citizens, businesspeople and government representatives who work together to ensure water quality in the Lititz Run Watershed.

To access the Millport Conservancy, exit the 222 freeway at the Akron exit, heading west on Newport Road Rte. 772. towards Lititz. In Rothsville, bear left at the light onto Rothsville Road. After a mile the road curves along the head of the valley - look the for sign pictured above for the left turn onto Warwick Road. The Millport Conservancy will be on your right all the way down the valley to Millport Road.
Enjoy the day!
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For Lancaster PA homes for sale click here. Jeff Geoghan is a top real estate expert in Lancaster County, PA, and an involved community member. Jeff's work has been featured in the Lancaster Newspapers, WGAL Channel 8, PA Business Journal and Wall Street Journal. Jeff's blog on Lancaster County and its homes is nationally-featured. Contact Jeff for more help with your Lancaster PA Real Estate needs.
Jeff, These pictures are so beautiful. I am so glad that this beautiful are in Lancaster County, PA is being preserved.